After losing to a widow in a high stakes casino game, Fujiko has to take on her request. She wants the gun of the man who killed her husband, so Fujiko finds herself going after the infamous gunman Daisuke Jigen.

The king of Austrian's grandchildren board a train carrying highly valued artworks, and to get to her target Fujiko impersonates herself as Maria, their tutor. While on board, she runs into a mysterious samurai from the East - Goemon Ishikawa.

Lupin the Third has declared that he will steal the mask of a famous opera singer. Inspector Zenigata takes note of this challenge, and decides to personally attend the singer's next performance.

As Lupin attempts to woo Fujiko, she tells him about a jewel peacock at the bottom of a pyramid in Egypt. Meanwhile, Jigen, who is short on cash after being scammed, goes on a search for the peacock as well. Jigen soon finds himself fighting against not only Lupin, but the various traps set up by looters. After both Lupin and Jigen fall into a trap together, Fujiko appears revealing the peacock will appear if one of them dies in the trap. However, they both manage to escape by sacrificing some scorpions. The peacock reveals itself, but Fujiko is forced to give it up in order to escape from the pyramid.

Fujiko is after the pendant belonging to a student in an all girls school. To get closer to her target, she impersonates herself as one of the teachers in the school.

Fujiko and Goemon become involved in a case that could very well lead to World War III.

Fortune-teller Shitoto can guess the "Death Date" of any person. The victims are all former marks of Lupin III. Jigen is hired to destroy the lithograph Shitoto uses to read these fortunes. Lupin teams up with Jigen, however, Lieutenant Oscar is waiting for him.

Lupin and Jigen find themselves looking after a girl who was used as a living piece of art, whilst evading Fujiko, who desperately wants to kill her for personal reasons.

Lupin investigates a group of owl people who desire Fujiko and begins to learn more about Fujiko's past whilst fighting off illusions brought on by a strange drug.

As Goemon tries to look after Fujiko, Oscar disguises himself as Fujiko and frames her for various perfect crimes in an attempt to gain Zenigata's favour.

Fujiko, Zenigata, Lupin, and Jigen go to a strange theme park to finally confront Fujiko's past, and the deranged Count Luis Yew Armied.

Fujiko enters a chamber where Count Luis Yu Almeida is sitting and waiting for her, however Lupin interferes from a communications room and being gunned by Jigen, who inhaled the Fraulein Eule Cult drug, which makes Lupin flee. Fujiko falls into another chamber where message appears in blocks and Lupin assists Fujiko to reach again the top. In a special room, Lupin explains the real events that occurred in the tragedy of Eulenspiegel and the process of brutally inducing mind control to little girls. Also in the room lies Dr. Kaiser's daughter Aisha who has been long time experimented for the sake of Count Almeida and now frail and weakened. Fujiko reveals that she entered the manor as a maid and intends to steal the Fraulein Eule cult drug, however she's been cornered by Almeida's men and experimented her as well. Her drive to steal and indulge in sex made Aisha somewhat happy.